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Method for purification of covalently closed DNA-m

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专利名称:Method for purification of covalently closed

DNA-molecules

发明人:Wittig, Burghardt,Junghans, Claas,Schroff,

Matthias

申请号:EP99250187.4申请日:19990614公开号:EP0967274A2公开日:19991229

摘要:the invention relates to a method of hantelf\örmige expressionskonstruktefrom plasmid dna can be made.the rumpfmolek\üle as parts of plasmids are moreisolated, the hantelf\örmigen molecules by ligation with oligodesoxyribonukleotidensynthesized and by subsequent sequenzspezifischen verdau of unwanted by-products byendo - and exonukleasen cleaned.the invention also concerns a method for cleaning thenukleins\äurepr\äparationen in biotechnological processes.

申请人:Mologen GmbH

地址:Fabeckstrasse 30 14195 Berlin DE

国籍:DE

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